other:
Expressing:Article Title: Synaptic plasticity in human thalamocortical assembloids
Article Snippet: .. Specifically, six assays were performed: (1) Colonies were immunostained for six pluripotency markers (Stemlight Pluripotency Antibody Kit 9656S, Cell Signaling); (2) Expression of five additional pluripotency markers was assayed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (3) G-banding; (4) Copy number variation at the seven most frequently aberrant chromosomal locations – was assayed using a custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (5) Global methylation analysis (Infinium MethylationEPICv1.0 850K Beadchip) was performed to identify methylation status at select epigenetic markers, , and results were then compared to established and previously published human embryonic stem cell and hiPSC lines ; (6) Trilineage assay (STEMdiff Trilineage Differentiation Kit 05230, STEMCELL Technologies) was performed, and markers of interest were analyzed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher). ..
Article Title: Synaptic plasticity in human thalamocortical assembloids
Article Snippet: .. Specifically, six assays were performed: (1) Colonies were immunostained for six pluripotency markers (Stemlight Pluripotency Antibody Kit 9656S, Cell Signaling); (2) Expression of five additional pluripotency markers was assayed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (3) G-banding; (4) Copy number variation at the seven most frequently aberrant chromosomal locations – was assayed using a custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (5) Global methylation analysis (Infinium MethylationEPICv1.0 850K Beadchip) was performed to identify methylation status at select epigenetic markers, , and results were then compared to established and previously published hiPSC lines; ; (6) Trilineage assay (STEMdiff Trilineage Differentiation Kit 05230, STEMCELL Technologies) was performed, and markers of interest were analyzed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher). ..
Quantitative RT-PCR:Article Title: Synaptic plasticity in human thalamocortical assembloids
Article Snippet: .. Specifically, six assays were performed: (1) Colonies were immunostained for six pluripotency markers (Stemlight Pluripotency Antibody Kit 9656S, Cell Signaling); (2) Expression of five additional pluripotency markers was assayed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (3) G-banding; (4) Copy number variation at the seven most frequently aberrant chromosomal locations – was assayed using a custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (5) Global methylation analysis (Infinium MethylationEPICv1.0 850K Beadchip) was performed to identify methylation status at select epigenetic markers, , and results were then compared to established and previously published human embryonic stem cell and hiPSC lines ; (6) Trilineage assay (STEMdiff Trilineage Differentiation Kit 05230, STEMCELL Technologies) was performed, and markers of interest were analyzed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher). ..
Article Title: Synaptic plasticity in human thalamocortical assembloids
Article Snippet: .. Specifically, six assays were performed: (1) Colonies were immunostained for six pluripotency markers (Stemlight Pluripotency Antibody Kit 9656S, Cell Signaling); (2) Expression of five additional pluripotency markers was assayed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (3) G-banding; (4) Copy number variation at the seven most frequently aberrant chromosomal locations – was assayed using a custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (5) Global methylation analysis (Infinium MethylationEPICv1.0 850K Beadchip) was performed to identify methylation status at select epigenetic markers, , and results were then compared to established and previously published hiPSC lines; ; (6) Trilineage assay (STEMdiff Trilineage Differentiation Kit 05230, STEMCELL Technologies) was performed, and markers of interest were analyzed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher). ..
Methylation:Article Title: Synaptic plasticity in human thalamocortical assembloids
Article Snippet: .. Specifically, six assays were performed: (1) Colonies were immunostained for six pluripotency markers (Stemlight Pluripotency Antibody Kit 9656S, Cell Signaling); (2) Expression of five additional pluripotency markers was assayed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (3) G-banding; (4) Copy number variation at the seven most frequently aberrant chromosomal locations – was assayed using a custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (5) Global methylation analysis (Infinium MethylationEPICv1.0 850K Beadchip) was performed to identify methylation status at select epigenetic markers, , and results were then compared to established and previously published human embryonic stem cell and hiPSC lines ; (6) Trilineage assay (STEMdiff Trilineage Differentiation Kit 05230, STEMCELL Technologies) was performed, and markers of interest were analyzed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher). ..
Article Title: Synaptic plasticity in human thalamocortical assembloids
Article Snippet: .. Specifically, six assays were performed: (1) Colonies were immunostained for six pluripotency markers (Stemlight Pluripotency Antibody Kit 9656S, Cell Signaling); (2) Expression of five additional pluripotency markers was assayed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (3) G-banding; (4) Copy number variation at the seven most frequently aberrant chromosomal locations – was assayed using a custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher); (5) Global methylation analysis (Infinium MethylationEPICv1.0 850K Beadchip) was performed to identify methylation status at select epigenetic markers, , and results were then compared to established and previously published hiPSC lines; ; (6) Trilineage assay (STEMdiff Trilineage Differentiation Kit 05230, STEMCELL Technologies) was performed, and markers of interest were analyzed using a Custom TaqMan RT-qPCR assay designed in-house (manufactured by ThermoFisher). ..
Immunofluorescence:Article Title: Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4 G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)
Article Snippet: α-Tubulin (Western Blot) , Rabbit anti- α-Tubulin (HRP conjugated) , 1:5000 , (Cell Signaling Technology Cat# 9099, RRID:AB_10695471). .. Pluripotency Markers (Immunofluorescence) , Rabbit anti-OCT4 , 1:300 , (Cell Signaling Technology Cat# 2890, RRID:AB_2167725). .. Pluripotency Markers (Immunofluorescence) , Mouse anti-SOX2 , 1:500 , (BD Biosciences Cat# 561469, RRID:AB_10694256).
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